A research team headed by Gražvydas Lukinavičius at the Max Planck Institute (MPI) for Biophysical Chemistry in Göttingen has now succeeded to improve fluorescent dyes for research. By combining the new dyes with 3D STED microscopy, the scientists achieved to resolve tiny structures in living cells such as microtubules with a diameter of about 23 nanometers.
Many scientific studies rely on fluorescent probes for highlighting specific structures in cells. For a long time, immunofluorescence of fixed cells and tissues dominated the field of fluorescence microscopy leading to the development of compatible fluorescent dyes. Recently, however, more attention is directed towards imaging of living cells. This imposes new restrictions on fluorescent dyes – they have to be cell-permeable and should not…